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Uppsala-Sweden/7 September 2009
From 2009.igem.org
Contents |
Plasmid Preps
Plasmid Prep with Sigma Gene Elute was performed with the following samples:
Subunit E2
- E21-1
- E22-3
- E23-3
- E24-1
Subunit E1 alpha
- E1A1-1
- E1A1-2
- E1A2-2
- E1A2-3
- E1A3-1
- E1A4-2
Subunit E1 beta
- E1B1-3
- E1B2-3
- E1B3-1
- E1B4-2
B0015, B0034-pirA, R0011-B0034, B0034-Yadh2-B0015
- T-1 - inoculation failed, new inoculation was performed
- RP-2
- RR-4
- RR-5
- RYT-1
- RYT-2
Furthermore these were prepped (Lux System etc.)
A340620, C0621, J237034
- A340620
- C0621
- J237034
and these here:
- R0062 (A)
- RARP 4, 5 ,7 (CH)
Plasmid Prep with Sigma Gene Elute was performed with the following samples:
RBS-pdc-RBS-ADH2(z) Three plasmid preps were performed for the large construct RBA-pdc-RBS-ADH2(z). With the final concenttations Media:Plasmidprep_090907.RTF
The result was evaluated by cutting with EcoR1 and PstI and running on a gel.
--Flormane 06:27, 8 September 2009 (UTC)
--Karl.brune 14:12, 7 September 2009 (UTC)
Report for the above preps: Media:Plasmidprep_090907.txt --Anders 17:49, 7 September 2009 (UTC)
2nd Plasmid Prep
Prep of the B0015 (AC3) culture from this morning
--Karl.brune 21:50, 7 September 2009 (UTC)
Pouring Plates
I poured plates ( Kanamycin and Chloramphenicol ), stored at iGEM fridge
--Karl.brune 21:50, 7 September 2009 (UTC)
Inoculation for PP
- pSB1A3
- pSB1AC3 (2)
- pSB1AK3 (2)
- B0034 (A) (2)
- I13504 (A) (2)
Overnight at 37C, 250RPM in 6ml LB with specific antitbiotic
--Karl.brune 21:50, 7 September 2009 (UTC)
