Template:Team:KULeuven/5 August 2009/VanillinProduction

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Miniprep of pairs:

  • RBS + Sam5 conc: 101,3 ng/ul
  • RBS+Sam8 conc: 95.7 ng/ul
  • Sam8 conc: 81,8 ng/ul
  • ComT , conc: 122,9 ng/ul


Restriction digestion
A1: Sam8, EcoRI en SpeI
A2: Sam5, EcoRI en XbaI

B1: RBS, EcoRI en SpeI
B2: ComT, EcoRI en XbaI

C1: Fcs, EcoRI en SpeI
C2: Ech, EcoRI en XbaI

The mixture was incubated in 37°C for 1h30...

...followed by gelelectrophoresis of the digested parts

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