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Team:KULeuven/3 August 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
[edit] Vanillin Production
We received the COMT, sam5 and sam8 from Edinburgh today. Unfortunately, the tube containing the sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.
- sam5 + RBS in (with PstI site removed),
- sam8 + RBS in ,
- sam8 in coding sequence,
- COMT in coding sequence,
The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so we can miniprep them tomorrow.
[edit] Vanillin Receptor
- Agrobacterium tumefaciens strain pTiA6NC/A1011 was plated out on LB-medium.
- Primers for virA, virG, promotor region virB and RpoA have arrived
