Team:Groningen
From 2009.igem.org
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Environmetal
- It all starts with arsenic in solution.
- Metal transport
- Metal accumulation
- Gas Vesicle
- Metal sensitive promoters
Heavy metal pollution in water and sediment endangers human health and the environment. To battle this problem a purification strategy, in which arsenic, zinc and copper are removed from water and sediment, was developed. This strategy encompasses a biological device in which E. coli bacteria accumulate metal ions from solutions, after which they produce gas vesicles and start floating. This biological device consists of two integrated systems: one for metal uptake and storage, the other for metal induced buoyancy. The uptake and storage system consists of a metal transporter and metallothioneins (metal accumulators). The buoyancy system is made up of a metal induced promotor in front of a gas vesicle gene cluster. The combination of both systems will enable the efficient cleaning of polluted water and sediment in a biological manner.
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