Categories :

Home Team Sponsors Parts Submitted to Registry Image Gallery Leave a Message!

Project :

Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

Notebook :

Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10

Team Logistics :

Protocols Stock List Research Shared Links and Help

Week 9

Monday

Tuesday

Wednesday

Wet Work

Threshold Devices

Confirmed successful ligation of pBad and I746350, I746351, I746352.

Thursday

Wet Work

Threshold Devices

Attempted the following standard assemblies:

pBad + I746350 to B0015

pBad + I746351 to B0015

pBad + I746352 to B0015

I746351 to B0015

I746352 to B0015

I746352 to B0015

The first three will be used to construct a complete device, with pBad as the sensor promoter and a pigment operon as the pigment-generating device. The pigment we chose for our proof of concept will be placed downstream of each of the 5 phage promoters, and then combined to give 15 combinations of activators and promoters, giving the construction below:

The second three will be used to construct a library of threshold devices which can be abstracted to black boxes with PoPS in and PoPS out:

Friday