Team:Newcastle/Labwork/10 September 2009

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Lab Work - 10/09/09

Metal Sensing Team

Introduction

Yesterday saw the Metal Sensing team attempt to transform DH5-alpha E.coli bacterial cells with two BioBricks (the cotC-GFP-smtA BioBrick and the kinA BioBrick) and plate them out on LB + kanamycin plates. The cotC-GFP-smtA BioBrick is part of the Metal Sensing team's sub-project and the kinA BioBrick will be used by the Stochastic Switch team's sub-project.

Today's exercise should see the Metal Sensing team inoculate LB medium with any transformant cultures which grow on these plates - if there are no cultures present then the transformations will be attempted a second time.

Observations

On all four plates there could be seen hundreds of transformant colonies - this was to be expected as the DNA used to transform the DH5-alpha cells was in pure form.

Procedure



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