Team:Groningen/Project

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[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
The Project
Gas Vesicles
Metal transport
Metal sensing
Metal storage

Planning and requirements:

  • Gasvesicle:
    • Modelling:
      • Bouyancy
        • Permeability
        • number of gasvesicles
        • Where do the gasvesciles end, in hight.
      • Mass of the bacteria (e.coli)
      • How long it takes before it floates
        • how long it take untill it is being expressed
        • How long it will take untill there are enough gasvesicles
        • how does it stay floating
    • Lab
      • GvP
        • Cluster of biobricks
        • Vector ordered from article
        • first expression with constutatieve promotor, later with metal sensitive promotor
      • Measurements
        • Where are the bacteria, what are bac concentrations on a certain hight
          • Making pictures on certain hight and compare with picture of known concentration
  • Metal importers
    • Modelling
      • Import speed
      • Amount
      • Max
    • Lab
      • HmtA
        • Zn/Cu alone
        • B-type ATPase (could be use if there is a ATP shortage?)
      • CitM
        • Divalent ions
        • Citrate around
        • Citrate can bind metals that are already bound.
      • Measurements
        • Transporter, on/off mechanism, up to what concentration (in the cell) does it still have metal uptake.
        • Measure concentration of metal. difference between begin and end concentrations of metal outside the cell
  • Promotors
    • Modelling
      • How do they react to metal, metal concentration vs expression of the protein after promotor (bv GFP)
    • Lab
      • Measuments
        • Expression of the gene behind the promotor(GFP)
      • Cu-promotor
      • Arc promotor
  • Accumulation
    • Modelling
      • Speed
      • Metaliotheines concentration
    • Lab
      • Measurements
          • Measure accumulation. By measuring before/after concentration metal with and without accumulation protein.
      • Biobrick Bba_K129004
      • Rest


Order of action

  1. Bouyancy
  2. Metal importation
  3. Accumulation
  4. Metal sensitive promotor