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Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation
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Revision as of 10:56, 30 September 2009 by
Droche
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Calibration protocols (CA)
Section
Assay
Overview and Aims
2.Auto-Induction
IPTG
Characterise Lac promoter by varying concentrations of IPTG
Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell