EPF-Lausanne/24 September 2009

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24 September 2009




Wet Lab

Transformation in Trp K.O.

The 3 newly competent strains (JW4356-2, JRG 1046, JRG 465) will be transformed with :

- RO2#4 + BB1 (+Amp, Kana) -> double transformation.

- RO2#4 (+Kana)

- RO1#1 (+Kana)

- RO1#1 + BB1 (+Amp, Kana) -> double transformation.

Note : the 3 control (1 ug/ul DNA, 100 pg/ul DNA, 10 pg/ul DNA -> 10ul for each transf) + KANA. We will count the nb of clones the next morning on the plates to determine transformation efficiency.

People in the lab


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