Team:Newcastle/Labwork/23 September 2009

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Lab Work - 23/09/09

Stochastic switch team

Today we discovered that the transfrormations seemed sucessful, and the control plates had no growth at all which is what was hoped for. Hopefuly this means that we have a transformed ara-sspb-pSB1AT3 ligation product.

  • We set up miniprep cultures for 12 colonies using LB + amp + tet
  • We also set up 12 more miniprep cultures for the Sac transformed cells in hope that the precious minipreps had been carried out incorrectly.



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