"
Template:Template HD FirstPage
From 2009.igem.org
Design your own promotor
As one of the most important approaches to synthezise synthetic promotors we developed a database that predicts the position of conserved promotor binding sequences. We coded an easy to use interface that is available for public use.
Start DesignMission 2009
Mammalian synthetic biology has huge potential, but it is in need of new standards and of systematic construction of comprehensive part libraries. Promoters are the fundamental elements of every synthetic biological system.
Mission accomplished
We have developed and successfully applied two novel, in silico guided methods for the rational construction of synthetic promoters and combined them with our targeted fluorescent protein tags.
iGem Heidelberg Mission 2009: Spybricks
Establishing new standards for iGEM, the Heidelberg 2009 team will be concerned with developing ways for measuring promoters in mammalian cells, a default chassis and a first evaluation of the recently postulated BioBrick beta proposal 2 (Tom Knight). Considering the importance of controlling gene expression, our team's work will focus on natural and synthetic mammalian promoters. Our vision is to provide the synthetic biology community with a methodical library of such promoters (with different output strength and sensitivity to different regulatory proteins) and a model which can provide guidance for the development of further synthetic promoters. Our efforts will therefore, from the very beginning, equally entail bioinformatics and wet lab work.
Team
Thirteen students and eleven advisors are working on this four month project. We split up into several subgroups whos focus and results you can follow on the Notebook and Results page. If you want to know more about the subgroups and the people involved, meet us on our Team page and let's get to know each other better at the Jamboree in Boston.
The Heartbeat Database
As one of the most important approaches to synthezise synthetic promotors we developed a database that predicts the position of conserved promotor binding sequences. We identified conserved sequences ourselves, analyzed the data and coded an easy to use interface that ise available for public use.
Notebook
What's about documenation? You will find the entire progress of the project and all the important steps on our Notebook page. The Notebook is divided into the individual subgroups.
Parts
Our team submits a library of well characterized and standardized promotors and sensors for eukaryotic cells. We will also contribute the first eucaryotic standart chassis for iGem featuring standardized genome integration sites.
Gallery
Spy on our cells or join the Heidelberg Team in the lab with our gallery tour.
Sponsors
This year we have a lot of distinguished sponsors, which support us and this project.
The Team
This year 13 students started the Heidelberg iGem team.
