Keep track with what we did so far

(29.08.09)

This eighth week of wetlab we have done the following things:

This seventh week of wetlab we have done the following things:

Beginning of the RbphP project (PCR colony)
Try of 1.5 step PCR to get the complete biobrick from LovTap -> didn't work
Check (digestion assay) to confirm we have the LovTap-term
Try of medium M9 for RO2
Characterisation (fluorescence in fonction of induction) for RO2
From LovTap-term, ligation to obtain the biobrick LacI-Rbs-LovTap-term with cloning steps (not from the 1.5 step PCR)

This sixth week of wetlab we have done the following things:

Protocol of ligation was refined.
!!! Inducible LOVTAP-term and Read out 2 biobrick were created.!!! The system is completed.
They need to be sequenced, characterized and submitted to the registery.

This fifth week of wetlab we have done the following things:

This fourth week of wetlab we have done the following things:

Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.

This third week of wetlab we have done the following things:

Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
Modeling part: Preliminar simulation was launched over the weekend.

This second week of wetlab we have done the following things:

Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
Modeling part: Files required to launch simulations were created and analyzed.

This first week of wetlab we have done the following things:

Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
Ordered and received the primers needed for the PCR of LovTAP
Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
Fused the two BioBricks "LacI" and "RBS"
Digested the LovTAP PCR products and RBS part