Team:Newcastle/Project/Labwork/Stocks

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Revision as of 21:43, 12 October 2009 by MJR09 (Talk | contribs)


Lab Stocks for Team:Newcastle


Team Newcastle 2009 iGEM 25-08-09 IMG 0529.JPG


BioBrick stocks in -20 degree freezer

Newcastle Stocks.jpg


















BioBrick DNA
Number BioBrick Description Plate/Well Plasmid Resistance on Plasmid In Eppendorf? Location Now (as purified plasmid) Location Now (as frozen transformed cells)
1 BBa_I13522 GFP Plate2/ Well 8A pSB1A2 Amp yes -20 freezer, iGEM space -86 freezer TPA 2458
2 BBa_J04450 RFP Plate 1/ Well 15A pSB1AT3 Amp, Tet yes -20 freezer, iGEM space -86 freezer TPA 2459
3 BBa_C0056 cI Repressor Plate1/Well 24A pSB1A2 Amp yes -20 freezer, iGEM space -86 freezer TPA 2460
4 BBa_B1002 Terminator sequence -20 freezer, iGEM space,Pink Rack, Top Right Hand side
5 BBa_C0077 CinR activator -20 freezer, iGEM space,Pink Rack, Top Right Hand side
6 BBa_C0076 Auto inducer synthetase -20 freezer, iGEM space,Pink Rack, Top Right Hand side
7 BBa_R0077 Promoter (CinR and HSC regulated RBS+) -20 freezer, iGEM space,Pink Rack, Top Right Hand side

TPA stocks in -86 degree freezer

BioBrick DNA
Strain Number TPA Chromosome sp. Date Frozen Initials Chromosome Gentotype
2458 E.Coli 30/07/2009 M.R. pSB1AK3 BBa_I13522 DHS-alpha
2459 E.Coli 30/07/2009 M.R. pSB1AT3 BBa_J04450 DHS-alpha
2460 E.Coli 30/07/2009 M.R. pSB1A2 BBa_C0056 JM109
2461 E.Coli 30/07/2009 M.R. pSB1AK3 BBa_B1002 JM109
2462 E.Coli 30/07/2009 M.R. pSB1A2 BBa_R0077 JM109
2463 E.Coli 30/07/2009 M.R. pMUTIN4 DHS-alpha



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