Team:TUDelft/RiboKeyLock Generator

From 2009.igem.org

Ribo Key & Lock Generator

Enter RBS DNA sequence:
example: TCACACAGGAAACC
or select a BioBrick RBS:




RBS RNA sequence:
example: UCACACAGGAAACC

Key RBS reverse complement RNA sequence with two mismatches:


Lock RBS reverse complement RNA sequence with one mismatch:



Key RNA sequence:

Lock RNA sequence:

Jump to mfold


Key DNA sequence:

Lock DNA sequence:



Key variables:

Upstream

Downstream

Further Downstream (optional part)


Position 1 (bp) of mismatch in the reverse complment of RBS


Position 2 (bp) of mismatch in the reverse complment of RBS



Lock variables:

Upstream

Scar + Start Codon

Mismatch sequence

Scar complement

Position (bp) of mismatch in the reverse complment of RBS


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