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Team:Brown/Notebook Meetings/8-3-09
From 2009.igem.org
iGEM Meeting 8-3-09
SFH 218
- 9:06: UTRA poster presentation. Call Kinko’s and let them know. Or contact Judy Nathanson for printing next door for much cheaper.
- 9:08: Gary will be out Thursday to Thursday. Poster practice on Wednesday.
- 9:13: Team 1 update
- EV131 is in pNoTat
- Making BL21 competent – need T7 polymerase; greater control over gene expression
- When running protein gel, run control cell w/o bacteria. To purify protein, will get 6-His antibodies and nickel columns.
- Possible binding assays – run against stationary histamine. Running free histamine.
- Threshold level of allergic rhinitis discussed.
- WE SHOULD INCLUDE ON POSTER the affinity information about EV131
- Also include considerations.
- What next after we get EV131 binding? Gary belabors.
- 9:40: Team 2 update
- Assembled Sec-GFP. Waiting on primers to amplify and drop into expression vector
- Also building Trg-EnvZ
- Working on making S. epi electrocompetent
- Timeline: make electrocompetent S. epi. Transform with expression vector. By end of week have expression in S. epi.
- Get a picture of S. epi on the microscope. Screen for contaminants.
- Also ordering primers for ccdB.
- 9:46: Gary’s advice about gel extractions
- Either run multiple lanes and combine, or run a big well
- 9:55: Team 3 update
- Now working with RFP as a selection marker
- Now using assembly service to build OmpC-RBS-RFP-DT. We need to focus on Taz1 mutation.
- Testing mutagenic primers this week.
- 10:06: Wednesday at 3PM. Poster practice.
