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Team:Newcastle/IntroductoryLabwork/6 July 2009
From 2009.igem.org
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Introductory Lab Session: 6th July 2009
List of bricks for the day
- [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (plasmid:pSB1AK3). Location: Plate 1, Well 13A
- [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] (plasmid:pSB1AK3). Location: Plate 2, Well 8A
- [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (plasmid:pSB1AT3). Location: Plate 1, Well 15A
Preparation of the tubes
- We got 3 tubes containing cells from the freezer at 80C and placed them into the ice and waited for 30 minutes
- We prepared 6 tubes (3 tubes for controls and 3 tubes for the plasmids) for the bricks
- We added 40ul of cells to each of the six tubes
- We added 3ul of DNA for the plasmid tubes and 3ul of water for the control tubes
- We added 900ul of LB for each of the six tubes
- We then placed the tubes in the shaking incubator for an hour.
Plating out
We prepared 9 plates with full strength (undiluted) and 9 plates with diluted tube content.
- Plates with undiluted content
- The first three plates having LB + Amp were used as the negative control. Hence the cells not having amp. resistance would not grow on these plates.
- The next three plates with LB content were used as the positive control. Cells without amp. resistanec would still grow on these plates.
- The other three plates were used for the plasmids.
| LB + Amp, (-) control 1 | LB, (+) control 1 | LB + Amp, Transformation Plasmid 1 |
| LB + Amp, (-) control 2 | LB, (+) control 2 | LB + Amp, Transformation Plasmid 2 |
| LB + Amp, (-) control 3 | LB, (+) control 3 | LB + Amp, Transformation Plasmid 3 |
- Plates with diluted content
- For the diluted ones we used 900ul of LB and 100ul of DNA from the corresponding tubes.
- The same set of 9 plates were prepared for the diluted content.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
