"
Uppsala-Sweden/1 September 2009
From 2009.igem.org
Transformations
Today we re-transformed the previously failed transformations (RBS-pdc-RBS-adh2 Z, RBS-adh2 Y and adh2 Y)
Digestion and ligation were performed according to the files below.
Media:Digestion_measure_090901.txt
Media:Ligation voulume calcuator_pSB1A3_ADH2Y_EP_090825.xls
Media:Assembly volume calcuator_RBS-adh2Y_090901.xls
Media:Assembly volume calcuator_RBS-pdc-RBS-adh2z_090901.xls
Plasmid preparation
Plasmid preps were performed for the inoculated cultures from yesterday. (RBS-PirB-T, pPetE-RBS, RBS B0034, F1610, R0011, F2622)
The results from DNA measurement was: Media:Plasmidprep_090901.txt
Glycerol stocks
Glycerol stocks (15%) was also made from the cultures inoculated yesterday. (RBS-PirB-T, pPetE-RBS, RBS B0034, F1610, R0011, F2622).
