Uppsala-Sweden/31 August 2009

From 2009.igem.org

Adh2Y PCR

Colony PCR of Transformations (RBS+pdc+RBS+adh2z (RPRB), pPetE+RBS, RBS-pirB-Ter

Colony PCR of Transformations (RBS+pdc+RBS+adh2z (RPRB), pPetE+RBS, RBS-pirB-Ter with VF2 and VR primers in 10ml reactions. 50°C annealing for 45 sec, 72° elongation for 3 min, 40 cylces

With self ligated vector (pSB1A3) as control

Gel of Colony PCR

File:20090831 colonypcr-labelled.jpg

File:20090831 colonypcr p2-labelled.jpg

Gel of Adh2Y PCR with BBa extension primers on Bakers Yeast lysate (Wine yeast did not work). --Karl.brune 15:18, 1 September 2009 (UTC)

Transformation

Digestion of RBS and Adh2Y PCR product prior to ligation. Media:Digestion(excert)090831.xls

--Anders 13:20, 31 August 2009 (UTC)

Digestion and cleanup report, 100 times dilution: Media:digest_purification_measur_09831.txt

--Anders 15:11, 31 August 2009 (UTC)

Amounts to be used for Assembly: Media:Assembly volume calcuator090831_1.xls and Biobricking: Media:Ligation voulume calcuator_090831_1.xls

--Anders 16:11, 31 August 2009 (UTC)