User contributions
From 2009.igem.org
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- 11:19, 21 October 2009 (diff | hist) File:BLPresult1.png (uploaded a new version of "Image:BLPresult1.png") (top)
- 11:18, 21 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 22:50, 20 October 2009 (diff | hist) File:BLPResult2.png (uploaded a new version of "Image:BLPResult2.png") (top)
- 22:49, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Steps) (top)
- 22:49, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Steps)
- 22:48, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Steps)
- 22:35, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Goal)
- 12:20, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:19, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:18, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:18, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:17, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 23:28, 19 October 2009 (diff | hist) Template:Team:KULeuven/6 October 2009/VanillinProduction (top)
- 23:27, 19 October 2009 (diff | hist) N Template:Team:KULeuven/12 October 2009/VanillinProduction (New page: * Brought the many many tubes from the vanilla experiment to HPLC measurements) (top)
- 23:25, 19 October 2009 (diff | hist) N Template:Team:KULeuven/18 October 2009/VanillinProduction (New page: * Vanilla experiment take 3! ** Transfered 1:100 to new LB cultures containing tyrosine or ferrulic acid or vanillin *** All had a concentration of 5 mg/L, vanillin was used to determine i...) (top)
- 23:20, 19 October 2009 (diff | hist) N Template:Team:KULeuven/17 October 2009/VanillinProduction (New page: * Last vanilla experiment showed unclear results, repeat the experiment but now wait a longer time to see if anything happens * Liquid cultures from PEFT, EFT, PAMSCOT and SAMS) (top)
- 23:19, 19 October 2009 (diff | hist) Template:Team:KULeuven/10 October 2009/VanillinProduction (top)
- 23:18, 19 October 2009 (diff | hist) N Template:Team:KULeuven/10 October 2009/VanillinProduction (New page: * Vanilla experiment take 2 ** From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium ** Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube ** ...)
- 22:57, 19 October 2009 (diff | hist) N Template:Team:KULeuven/7 October 2009/VanillinProduction (New page: * Attempts to start another vanilla experiment failed due to lack of materials ** gathered materials and next attempt will be saturday) (top)
- 22:56, 19 October 2009 (diff | hist) N Template:Team:KULeuven/9 October 2009/VanillinProduction (New page: * Liquid cultures (for sending DNA to the registry) made from: ** EF ** EFT ** PEFT ** SAMS ** PAMS ** PAMSCO ** PAMSCOT **SAM5 **SAM8 **Ech **Fcs *Liquid cultures for the next vanilla exp...) (top)
- 22:52, 19 October 2009 (diff | hist) N Template:Team:KULeuven/6 October 2009/VanillinProduction (New page: * No colonies on both plates ** best to give up on PAMSCOEFT ligation at this point since the end is nearing)
- 22:52, 19 October 2009 (diff | hist) N Template:Team:KULeuven/5 October 2009/VanillinProduction (New page: * Double Electroporation of PAMSCOEFT (used extra high volume of ligation mixture, 5 μl instead of 2-3 μl)) (top)
- 22:51, 19 October 2009 (diff | hist) N Template:Team:KULeuven/3 October 2009/VanillinProduction (New page: * Miniprep of EFT and PAMSCO * Restriction digest of 4 EFT overnight * Restriction digest of 4 PAMSCO overnight) (top)
- 22:50, 19 October 2009 (diff | hist) N Template:Team:KULeuven/2 October 2009/VanillinProduction (New page: * Reattempt to ligate PAMSCOEFT ** made liquid cultures of PAMSCO ** made liquid cultures of EFT) (top)
- 22:49, 19 October 2009 (diff | hist) N Template:Team:KULeuven/4 October 2009/VanillinProduction (New page: * Gel electrophorese of EFT and PAMSCO * Gel extraction of EFT and PAMSCO {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | EFT || 1,5 || -7,...) (top)
- 22:46, 19 October 2009 (diff | hist) N Template:Team:KULeuven/5 october 2009/VanillinProduction (New page: * Double Electroporation of PAMSCOEFT (used extra high volume of ligation mixture, 5 μl instead of 2-3 μl)) (top)
- 22:41, 19 October 2009 (diff | hist) Template:Team:KULeuven/4 october 2009/VanillinProduction (top)
- 22:41, 19 October 2009 (diff | hist) N Template:Team:KULeuven/4 october 2009/VanillinProduction (New page: * Gel electrophorese of EFT and PAMSCO * Gel extraction of EFT and PAMSCO {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | EFT || 1,5 || -7,...)
- 22:33, 19 October 2009 (diff | hist) Template:Team:KULeuven/3 october 2009/VanillinProduction (top)
- 22:30, 19 October 2009 (diff | hist) N Template:Team:KULeuven/2 october 2009/VanillinProduction (New page: * Reattempt to ligate PAMSCOEFT ** made liquid cultures of PAMSCO ** made liquid cultures of EFT) (top)
- 22:30, 19 October 2009 (diff | hist) N Template:Team:KULeuven/3 october 2009/VanillinProduction (New page: * Miniprep of EFT and PAMSCO * Restriction digest of EFT overnight * Restriction digest of PAMSCO overnight)
- 22:28, 19 October 2009 (diff | hist) N Template:Team:KULeuven/29 September 2009/VanillinProduction (New page: * Nanodrop of the six cultures of PAMSCOEFT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PAMSCOEFT1 || 147,4 || || |- align="center" | ...) (top)
- 22:25, 19 October 2009 (diff | hist) Template:Team:KULeuven/28 September 2009/VanillinProduction (top)
- 22:24, 19 October 2009 (diff | hist) Template:Team:KULeuven/26 September 2009/VanillinProduction (top)
- 22:23, 19 October 2009 (diff | hist) N Template:Team:KULeuven/27 September 2009/VanillinProduction (New page: * Made liquid cultures of the 4 transfered cultures of PAMSCOT * Made liquid cultures of the 4 transfered cultures of PAMSCOEFT) (top)
- 22:23, 19 October 2009 (diff | hist) N Template:Team:KULeuven/26 September 2009/VanillinProduction (New page: * Transfered 4 colonies from the electroporation of PAMSCO to a new plate * Transfered 4 colonies from the electroporation of PAMSCOT to a new plate)
- 22:22, 19 October 2009 (diff | hist) Template:Team:KULeuven/28 September 2009/VanillinProduction
- 22:21, 19 October 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production (top)
- 22:20, 19 October 2009 (diff | hist) N Template:Team:KULeuven/28 September 2009/VanillinProduction (New page: * Miniprep of PEFT, PAMSCO electroporation, PAMSCOEFT electroporation {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PEFT1 || 240,3 || ||...)
- 21:51, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:43, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:42, 19 October 2009 (diff | hist) N File:BLPResult2.png
- 21:39, 19 October 2009 (diff | hist) File:BLPresult1.png (uploaded a new version of "Image:BLPresult1.png")
- 21:38, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:36, 19 October 2009 (diff | hist) N File:BLPresult1.png
- 21:07, 19 October 2009 (diff | hist) N Template:Team:KULeuven/25 September 2009/VanillinProduction (New page: * Electroporation of PAMSCOT * Electroporation of PAMSCOEFT * Miniprep EFT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | EFT1 || 79,8 || ...) (top)
- 21:02, 19 October 2009 (diff | hist) N Template:Team:KULeuven/24 September 2009/VanillinProduction (New page: * Made motherplate of PAMSCO * restriction digest of PAMSCO with E/S * Gel extraction PAMSCO restriction digest * Restriction digest of Terminator and EFT with E/X * Ligation of PAMSCO wit...) (top)
- 20:55, 19 October 2009 (diff | hist) N Template:Team:KULeuven/22 September 2009/VanillinProduction (New page: * Made 12 liquid cultures of PEFT cells * Made 12 liquid cultures of EFT cells * Liquid cultures of 6 PAMSCO ligation cells and transfered to a plate. * Glycerol stock of EFT, PEFT and SAM...) (top)
- 20:53, 19 October 2009 (diff | hist) N Template:Team:KULeuven/23 September 2009/VanillinProduction (New page: * Miniprep of PAMS&Co {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PAMSCO1 || 115,6 || || |- align="center" | PAMSCO2 || 102,2 || || |...) (top)
- 20:39, 19 October 2009 (diff | hist) N Template:Team:KULeuven/21 September 2009/VanillinProduction (New page: *Plating of Pams&co *Miniprepped electroporated PEFT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PEFT1 1 UV || 155,4 || 1,99|| |- ali...) (top)
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