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EPF-Lausanne/16 October 2009
From 2009.igem.org
Contents |
Wet Lab
Miniprep of liquid cultures containing the plasmids with the possible (though unpure) mutated LovTap gene.
Digestion assay of LovTap in iGEM plasmid (the one we just cloned these last days): we digested it with EcoRI and PstI. In theory we should obtain the cut plasmid, and 3 bands at respectively about 150, 250 and 350 bp. The gel photo confirming that our LovTap is correct is here:
Prepared a new M9 medium to do further tests on RO1 and RO2.
Tried to test RO2 with different concentrations of Trp and ATC and took measurements during a few hours with the qPCR machine, but this didn't give any results, as you can see in the following graphs:
Did an SDS-PAGE to test the level of expression of our LovTap biobrick with +/- IPTG added to the culture medium of the cells. Ran the gel, stained it, started destaining it. End of the protocol and photo were finished on Saturday.
People in the lab
Tu, Gab, Basile, Christian
