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EPF-Lausanne/3 August 2009
From 2009.igem.org
Contents |
Wet Lab
PCR of LacI-RBS, LOVTAP (to do again LOVTAP-TERM plasmid in case our clone hadn't the correct sequence), 1,5 step PCR (see lab notebook for details) to produce the whole desired part (LacI-RBS-LOVTAP-TERM, LacLP-RBS-LOVTAP-TERM, T7-RBS-LOVTAP-TERM), and finally a PCR of LOVTAP-TERM (with required plasmid) was done, to ensure having the right sequence for the LOVTAP-TERM.
People in the lab
Nath, Basile, Gab, Christian, Nicolas
