EPF-Lausanne/7 July 2009

From 2009.igem.org

(Difference between revisions)
(People in the lab)
(People in the lab)
 
(60 intermediate revisions not shown)
Line 2: Line 2:
<div CLASS="epfltrick">__TOC__
<div CLASS="epfltrick">__TOC__
</div><div CLASS="epfl09">
</div><div CLASS="epfl09">
 +
 +
<html>
 +
<body>
 +
<form action="input_button.htm">
 +
<p align="right">
 +
<input type="button" name="lien" value="6 July 2009"
 +
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/6_July_2009'">
 +
<input type="button" name="lien" value="8 July 2009"
 +
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/8_July_2009'">
 +
</p>
 +
</form>
 +
</body>
 +
</html>
 +
<html><center>
<html><center>
Line 39: Line 53:
==People in the lab==
==People in the lab==
:Tu, Heidi, Rafael, Basile, Nath
:Tu, Heidi, Rafael, Basile, Nath
-
 
-
<!--
 
-
 
-
<html><center><a href="https://2009.igem.org/EPF-Lausanne/6_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Fl%C3%A8che_gauche.png/70px-Fl%C3%A8che_gauche.png"></a></center></html> 
 
-
<html><center><a href="https://2009.igem.org/EPF-Lausanne/8_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html>
 
-
 
-
--->
 
-
 
-
<html>
 
-
<body>
 
-
<form action="input_button.htm">
 
-
<p>
 
-
<input type="button" name="lien" value="8 July 2009"
 
-
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/8_July_2009'">
 
-
</p>
 
-
</form>
 
-
</body>
 
-
</html>
 
 +
<html><center><a href="https://2009.igem.org/EPF-Lausanne/6_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> 
 +
<a href="https://2009.igem.org/EPF-Lausanne/8_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html>
</div><div CLASS="epfl09bouchon"></div>
</div><div CLASS="epfl09bouchon"></div>

Latest revision as of 08:49, 28 July 2009

Contents


7 July 2009




Wet Lab

We have to grow the 3 strains generously sent by Tom Beatty

The three strains are :

  • R.Palustris CEA001 (wild type) ; should be grown on LB medium only
  • R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
  • E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)


The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)


pUC19 plasmid


We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.

Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.


Cloning Strategy

To design plasmids : software Vector NTI


People in the lab

Tu, Heidi, Rafael, Basile, Nath