EPF-Lausanne/7 October 2009

Wet Lab

Prepared cells for time-course experiment:

- 7mL of fresh LB + 1mL of overnight cell culture (DH5 alpha RO2.4 + BB1 clone n.3)

- 5 different conditions:

         - +light +IPTG -Trp

         - +light -IPTG -Trp

         - -light +IPTG -Trp

         -  -light -IPTG +Trp

         -  -light -IPTG -Trp

The cells were put in the incubator at 37°C in their respective experimental conditions. Measurements of OD and fluorescence will be taken every 30 min starting at 1h of incubation, so that we have a time-course measurement. For the last sample, we will do a kinetic measurement overnight to see the decay of the RFP fluroescence.

Miniprep of the Trp-mutated strains that should be double-transformants (RO1.1+BB1 and RO2.4+BB1) to extract the DNA and make a digestion assay in order to confirm the gel's results. Also miniprepped DH5 alpha RO2.4+BB1 clone n.3 to extract the 2 working plasmids (LovTap and read-out 2), so that we can use this DNA directly for future transformations.

Results of the plate-reader experiment

071009_dh5alpha_ro2_dt.jpg

People in the lab

Gab, Tu