EPF-Lausanne/9 September 2009

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Contents

9 September 2009





Wet Lab

Characterization



With Atc Without Atc
With Trp
1/2 Trp + 1/2 Atc
1 Trp + 1/2 Atc
1/2 Trp + 1 Atc
1 Trp + 1 Atc
1/2 Trp
1 Trp
3/2 Trp
Without Trp 1/2 Atc
1 Atc
3/2 Atc
Without Atc nor Trp


Results of the characterization

For the RBS :

RBS plot

RBS was our negative control. We can see that there is no RFP fluorescence (because of course RBS has no RFP gene).

For LacI-RFP 1 + IPTG :

LacI-RFP + IPTG plot

LacI-RFP 2 + IPTG show the same tendancy.

RO1#1 + 0.5 TRP :

RO1#1 +0.5 TRP

RO1#2 + 0.5 TRP shows the same tendancy.

RO1#1 + 1 TRP :

RO1#1 + 1 TRP

Again, RO1#2 clone has the same behaviour.

RO1#1 without TRP:

RO1#1 - TRP

Clone #2 has the same behaviour.

People in the lab

Mélanie, Caroline, Basile, Nicolas