Results Recombinase

From 2009.igem.org

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[[Image:PTetFlp-KanR%2BF3-ZeoR-F3-RFP%2C_digested_with_PstI.JPG|500px|]]
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[[Image:Re-electroporated_pTetFlp-KanR%2BF3-ZeoR-F3-RFP%2C_digested_with_PstI.JPG|500px|]]
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[[Image:PMA-P_FRT_dhfr_00500bp.jpg|500px|]]
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[[Image:PMA-TT_FRT_GFP_BsdR.jpg|500px|]]

Latest revision as of 19:20, 16 October 2009

Recombinase - Results

And those pretty pictures were created, following this protocol.

PCR product of pR6K-KanR.JPG PRhaFlp-CmR digested with EcoRI .JPG Re-electroporation of pRhaFlp-CmR, digestedwith EcoRI .JPG PRhaFlp-CmR-KanR, digested with PvuII.JPG Re-electroporated pRhaFlp-CmR-KanR ,digested with PvuII.JPG PTetFlp-KanR, digested with Pst I.JPG Re-electroporation of pTetFlp-KanR with Pst I and EcoRI digest.JPG F3-ZeoR-F3-RFP digest with PvuII.JPG PTetFlp-KanR+F3-ZeoR-F3-RFP, digested with PstI.JPG Re-electroporated pTetFlp-KanR+F3-ZeoR-F3-RFP, digested with PstI.JPG PMA-P FRT dhfr 00500bp.jpg PMA-P FRT dhfr 01000bp.jpg PMA-P FRT dhfr 02000bp.jpg PMA-P FRT dhfr 05000bp.jpg PMA-RQ-P FRT dhfr 10000bp.jpg PMA-RQ-P F3 zeoR F3 RFP.jpg

PMA-TT FRT GFP BsdR.jpg
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