User contributions
From 2009.igem.org
- 03:57, 22 October 2009 (diff | hist) File:Uw js.txt (uploaded a new version of "Image:Uw js.txt") (top)
- 03:56, 22 October 2009 (diff | hist) File:Uw js.txt (uploaded a new version of "Image:Uw js.txt")
- 03:52, 22 October 2009 (diff | hist) File:Uw js.txt (uploaded a new version of "Image:Uw js.txt")
- 03:42, 22 October 2009 (diff | hist) File:Univers Washington Team photo.jpg (uploaded a new version of "Image:Univers Washington Team photo.jpg") (top)
- 03:31, 22 October 2009 (diff | hist) Template:Team:Washington/Templates/Header (top)
- 03:27, 22 October 2009 (diff | hist) Template:Team:Washington/Templates/Header
- 03:12, 22 October 2009 (diff | hist) File:Univers Washington Team photo.jpg (uploaded a new version of "Image:Univers Washington Team photo.jpg")
- 02:57, 22 October 2009 (diff | hist) Team:Washington/Team (→Who we are)
- 02:57, 22 October 2009 (diff | hist) Team:Washington/Team
- 02:52, 22 October 2009 (diff | hist) Team:Washington/Notebook/Microscope (top)
- 02:50, 22 October 2009 (diff | hist) Team:Washington/Notebook/Microscope (→Microscope)
- 01:30, 22 October 2009 (diff | hist) Team:Washington/Team (→Where we worked)
- 01:26, 22 October 2009 (diff | hist) Team:Washington/Team (→Who we are)
- 01:26, 22 October 2009 (diff | hist) Team:Washington/Team (→Who we are)
- 01:25, 22 October 2009 (diff | hist) N File:Univers Washington Team photo.jpg
- 01:10, 22 October 2009 (diff | hist) File:Rossetta match and Foldit.png (uploaded a new version of "Image:Rossetta match and Foldit.png") (top)
- 00:02, 21 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→Problem)
- 00:02, 21 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→Problem)
- 00:01, 21 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→Problem)
- 22:16, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt
- 21:51, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→The Idea)
- 21:13, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→The Idea)
- 21:13, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→The Idea)
- 21:13, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→The Idea)
- 21:12, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→The Idea)
- 21:12, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt (→The Idea)
- 21:11, 20 October 2009 (diff | hist) Team:Washington/Project/FoldIt
- 21:10, 20 October 2009 (diff | hist) N File:Rossetta match and Foldit.png
- 20:22, 20 October 2009 (diff | hist) Team:Washington/Accomplishments (→Synthesized and BioBricked type I secretion system from Erwinia chrysanthemi) (top)
- 20:22, 20 October 2009 (diff | hist) Team:Washington/Accomplishments (→Synthesized and BioBricked type I secretion system from Erwinia chrysanthemi)
- 20:22, 20 October 2009 (diff | hist) Team:Washington/Accomplishments (→Synthesized and BioBricked type I secretion system from Erwinia chrysanthemi)
- 05:16, 19 October 2009 (diff | hist) Team:Washington/Project/FoldIt
- 05:15, 19 October 2009 (diff | hist) Team:Washington/Project/Display
- 05:15, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 05:15, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 05:14, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 02:01, 19 October 2009 (diff | hist) Team:Washington/Bibliography (→Display)
- 01:59, 19 October 2009 (diff | hist) Team:Washington/Project (→Traditional Protein Purification vs Our System: Ideal Protein Purification)
- 01:56, 19 October 2009 (diff | hist) Team:Washington (→The Idealized Protein Purification System: Improving the lives of molecular biologists)
- 01:53, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 01:52, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 01:51, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 01:51, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 01:51, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 01:50, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Results)
- 01:32, 19 October 2009 (diff | hist) Team:Washington/Bibliography (→General overview)
- 01:29, 19 October 2009 (diff | hist) Team:Washington/Bibliography (→Target)
- 01:28, 19 October 2009 (diff | hist) Team:Washington/Project/Display (→Citations)
- 01:27, 19 October 2009 (diff | hist) Team:Washington/Bibliography (→Display)
- 01:26, 19 October 2009 (diff | hist) N Team:Washington/Bibliography (New page: __NOTOC__ {{Template:Team:Washington/Templates/Header}} ====General overview==== #Protein production and purification, Structural Genomics Consortium et.al. Nature Methods Vol.5 NO.2, Febr...)
- 01:13, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 01:09, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 01:08, 19 October 2009 (diff | hist) File:Secretion plas.PNG (uploaded a new version of "Image:Secretion plas.PNG") (top)
- 01:04, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 01:04, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 01:03, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 01:02, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 01:02, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 01:01, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 00:58, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 00:58, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 00:57, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 00:36, 19 October 2009 (diff | hist) Team:Washington/Project/Target
- 00:36, 19 October 2009 (diff | hist) Team:Washington/Project/Target
- 00:35, 19 October 2009 (diff | hist) Team:Washington/Project/Display
- 00:34, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Citations)
- 00:33, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 00:30, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 00:29, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Background)
- 00:25, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Results)
- 00:18, 19 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 23:46, 18 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Background)
- 23:34, 18 October 2009 (diff | hist) Team:Washington/Project
- 23:34, 18 October 2009 (diff | hist) Team:Washington/Project
- 23:32, 18 October 2009 (diff | hist) Team:Washington/Project (→Traditional Protein Production and Purification)
- 23:21, 18 October 2009 (diff | hist) Team:Washington/Project (→Citations)
- 23:18, 18 October 2009 (diff | hist) Team:Washington/Project (→Idealized Protein Production and Purification)
- 23:15, 18 October 2009 (diff | hist) Team:Washington/Project (→Citations)
- 23:14, 18 October 2009 (diff | hist) Team:Washington/Project (→Citations)
- 23:05, 18 October 2009 (diff | hist) Team:Washington/Project (→Traditional Protein Production and Purification)
- 22:59, 18 October 2009 (diff | hist) Team:Washington/Project
- 22:54, 18 October 2009 (diff | hist) Team:Washington/Project (→Traditional Protein Production and Purification)
- 22:53, 18 October 2009 (diff | hist) Team:Washington/Project (→Traditional Protein Purification vs Our System: Ideal Protein Purification)
- 22:40, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 22:37, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 22:35, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 22:30, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 22:19, 18 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Conclusion)
- 22:13, 18 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Constructing the Secretion System)
- 22:05, 18 October 2009 (diff | hist) Team:Washington/Accomplishments (→Developed assay to quantify protein secretion)
- 22:03, 18 October 2009 (diff | hist) Team:Washington/Accomplishments (→Worked on computationally designing novel Biotin-binding protein using Rosetta)
- 21:55, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 21:53, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 21:52, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 21:52, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 21:47, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 21:41, 18 October 2009 (diff | hist) Team:Washington/Future (→Current Focus)
- 21:40, 18 October 2009 (diff | hist) Team:Washington/Future
- 21:39, 18 October 2009 (diff | hist) Team:Washington/Future (→Fold-It)
- 21:37, 18 October 2009 (diff | hist) Team:Washington/Notebook (→Protocols)
- 21:36, 18 October 2009 (diff | hist) Team:Washington/Notebook/Standard curve (top)
- 21:36, 18 October 2009 (diff | hist) Team:Washington/Notebook (→Protocols)
- 21:35, 18 October 2009 (diff | hist) Team:Washington/Notebook (→Protocols)
- 21:31, 18 October 2009 (diff | hist) Team:Washington/Notebook/Standard curve (→Generating a Standard curve for GFP concentration)
- 21:18, 18 October 2009 (diff | hist) Team:Washington/Notebook/Standard curve (→Generating a Standard curve for GFP concentration)
- 21:11, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 21:05, 18 October 2009 (diff | hist) Team:Washington/Future (→Fold-It)
- 21:04, 18 October 2009 (diff | hist) N Team:Washington/Notebook/Standard curve (New page: ====Generating a Standard curve for GFP concentration==== #Purify GFP-fused protein)
- 20:52, 18 October 2009 (diff | hist) Team:Washington/Project/Target (→GFP Insertion and Characterization)
- 20:48, 18 October 2009 (diff | hist) Team:Washington/Future (→Overview)
- 20:48, 18 October 2009 (diff | hist) Team:Washington/Future (→Current Focus)
- 20:47, 18 October 2009 (diff | hist) Team:Washington/Future (→Idea)
- 20:47, 18 October 2009 (diff | hist) Team:Washington/Future (→Idea)
- 20:47, 18 October 2009 (diff | hist) Team:Washington/Future (→Idea)
- 20:46, 18 October 2009 (diff | hist) Team:Washington/Future
- 20:45, 18 October 2009 (diff | hist) Team:Washington/Future
- 20:45, 18 October 2009 (diff | hist) Team:Washington/Future
- 20:44, 18 October 2009 (diff | hist) N File:PDS design.png (top)
- 20:43, 18 October 2009 (diff | hist) Team:Washington/Future (→Custom Display Vector)
- 20:42, 18 October 2009 (diff | hist) Team:Washington/Future (→New Display Vector)
- 20:28, 18 October 2009 (diff | hist) Team:Washington/Future (→New Display Vector)
- 20:28, 18 October 2009 (diff | hist) Team:Washington/Future (→New Display Vector)
- 20:12, 18 October 2009 (diff | hist) Team:Washington/Future (→New Display Vector)
- 19:59, 18 October 2009 (diff | hist) Team:Washington/Future (→New Display Vector)
- 19:57, 18 October 2009 (diff | hist) Team:Washington/Future
- 19:55, 18 October 2009 (diff | hist) Team:Washington/Future
- 19:54, 18 October 2009 (diff | hist) Team:Washington/Future (→The Trench Work)
- 19:52, 18 October 2009 (diff | hist) Team:Washington/Future (→Overview)
- 19:51, 18 October 2009 (diff | hist) Team:Washington/Future (→Current Focus)
- 19:51, 18 October 2009 (diff | hist) Team:Washington/Future (→Fold-It)
- 19:51, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-It)
- 19:50, 18 October 2009 (diff | hist) Team:Washington/Future
- 19:45, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:44, 18 October 2009 (diff | hist) m Team:Washington/Project/Display (→The Trench Work)
- 19:44, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:43, 18 October 2009 (diff | hist) File:Foldit 2.png (uploaded a new version of "Image:Foldit 2.png") (top)
- 19:41, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-It)
- 19:26, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-It)
- 19:25, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:25, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:25, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:24, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:23, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:23, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:22, 18 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 19:21, 18 October 2009 (diff | hist) Team:Washington/Project/Display
- 19:15, 18 October 2009 (diff | hist) N File:Foldit 4.png (top)
- 19:14, 18 October 2009 (diff | hist) N File:Foldit 2.png
- 22:45, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 22:45, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→The Trench Work)
- 22:44, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Problem)
- 22:30, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-It)
- 22:05, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:05, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:05, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:04, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:04, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:03, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:03, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 22:02, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 22:02, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 22:02, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→http://fold.it/portal/|Fold-it)
- 22:01, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→[http://fold.it/portal/]|Fold-it)
- 22:00, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 22:00, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→[http://fold.it/portal/|Fold-it])
- 22:00, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→[www.fold.it |Fold-it])
- 21:59, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→[www.fold.it|Fold-it])
- 21:59, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 21:58, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 21:57, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 20:56, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 20:54, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 18:14, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Fold-it)
- 17:59, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→References)
- 17:58, 17 October 2009 (diff | hist) Team:Washington/Project/Display (→Background)
- 17:58, 17 October 2009 (diff | hist) Team:Washington/Project/Display
- 00:48, 17 October 2009 (diff | hist) Team:Washington/Notebook/IMAC protocol
- 00:47, 17 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR (top)
- 00:46, 17 October 2009 (diff | hist) Team:Washington/Notebook/IMAC protocol
- 00:46, 17 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 00:45, 17 October 2009 (diff | hist) Team:Washington/Notebook/IMAC protocol
- 00:30, 17 October 2009 (diff | hist) Team:Washington/Notebook/IMAC protocol
- 23:33, 16 October 2009 (diff | hist) Team:Washington/Notebook
- 23:33, 16 October 2009 (diff | hist) Team:Washington/Notebook
- 23:32, 16 October 2009 (diff | hist) N Team:Washington/Notebook/IMAC protocol (New page: ====BioRad Micro Column Protein Prep Protocol====)
- 19:58, 16 October 2009 (diff | hist) Team:Washington/Notebook (→Protocols)
- 19:57, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:47, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:40, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:24, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:24, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:09, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:08, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:04, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:04, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:03, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 14:03, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR (→SOEing PCR)
- 02:07, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 02:06, 16 October 2009 (diff | hist) Team:Washington/Notebook/SOEingPCR
- 02:04, 16 October 2009 (diff | hist) Team:Washington/Notebook
- 02:03, 16 October 2009 (diff | hist) N Team:Washington/Notebook/SOEingPCR (New page: ====SOEing PCR==== #Design primers **VF2 / VR = standard forward and revers primer from original construct ##For mutations/insertions/deletions of 1-15 base pairs **Forward: 5'-----...)
- 07:35, 15 October 2009 (diff | hist) Team:Washington/Notebook (→Protocols)
- 07:35, 15 October 2009 (diff | hist) Team:Washington/Notebook (→Project Time Line)
- 07:27, 15 October 2009 (diff | hist) Team:Washington/Accomplishments (→Bio-Bricks)
- 07:27, 15 October 2009 (diff | hist) Team:Washington/Accomplishments (→Display System)
- 07:26, 15 October 2009 (diff | hist) Team:Washington/Accomplishments (→Secretion System)
- 07:26, 15 October 2009 (diff | hist) Team:Washington/Accomplishments (→Accomplished)
- 07:25, 15 October 2009 (diff | hist) Team:Washington/Accomplishments
- 07:18, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:14, 15 October 2009 (diff | hist) Team:Washington/Notebook (→Protocols)
- 07:13, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:12, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:12, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:11, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:10, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Experiments)
- 07:09, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:08, 15 October 2009 (diff | hist) File:Secretion plas.PNG (uploaded a new version of "Image:Secretion plas.PNG")
- 07:05, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:04, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:03, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion
- 07:02, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Secreting the Target Protein)
- 07:02, 15 October 2009 (diff | hist) Team:Washington/Project/Secretion (→Background)
- 06:59, 15 October 2009 (diff | hist) Team:Washington/Project/Target (→Background)
- 06:59, 15 October 2009 (diff | hist) Team:Washington/Project/Target (→Experiments)
- 06:58, 15 October 2009 (diff | hist) Team:Washington/Project/Target (→'Citations')
- 06:58, 15 October 2009 (diff | hist) Team:Washington/Project/Target (→Citations)
- 06:56, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→Flow Cytometry)
- 06:53, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→Data)
- 06:43, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→Harvard 2006 Surface Display System: Documented Data)
- 06:39, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→Background)
- 06:31, 15 October 2009 (diff | hist) Team:Washington/Project/Display
- 06:19, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→References)
- 06:18, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→Background)
- 06:08, 15 October 2009 (diff | hist) Team:Washington/Project
- 06:07, 15 October 2009 (diff | hist) Team:Washington/Project
- 06:05, 15 October 2009 (diff | hist) Team:Washington/Project
- 06:03, 15 October 2009 (diff | hist) Team:Washington/Project
- 06:02, 15 October 2009 (diff | hist) Team:Washington/Project
- 06:02, 15 October 2009 (diff | hist) Team:Washington/Project
- 06:01, 15 October 2009 (diff | hist) Team:Washington/Project
- 05:56, 15 October 2009 (diff | hist) File:Strept-biotin.png (uploaded a new version of "Image:Strept-biotin.png") (top)
- 05:51, 15 October 2009 (diff | hist) Team:Washington/Project/Display (→Background)
- 05:50, 15 October 2009 (diff | hist) N File:Strept-biotin.png (structure)
- 05:32, 15 October 2009 (diff | hist) Team:Washington/Accomplishments
- 05:31, 15 October 2009 (diff | hist) Team:Washington/Accomplishments
- 05:16, 15 October 2009 (diff | hist) Team:Washington/Future
- 05:15, 15 October 2009 (diff | hist) Team:Washington/Future
- 05:12, 15 October 2009 (diff | hist) Team:Washington/Notebook/NheI (top)
- 05:12, 15 October 2009 (diff | hist) Team:Washington/Notebook/NheI PstI (top)
- 05:12, 15 October 2009 (diff | hist) Team:Washington/Notebook/colony PCR (top)
- 05:12, 15 October 2009 (diff | hist) Team:Washington/Notebook/gene synthesis (top)
- 05:12, 15 October 2009 (diff | hist) Team:Washington/Notebook/2mL purification (top)
- 05:12, 15 October 2009 (diff | hist) Team:Washington/Notebook/Ni-column (top)
- 05:11, 15 October 2009 (diff | hist) Team:Washington/Notebook/50mL purification (top)
- 05:11, 15 October 2009 (diff | hist) Team:Washington/Notebook/Flow Cytometry
- 05:11, 15 October 2009 (diff | hist) Team:Washington/Notebook/Microscope
- 05:11, 15 October 2009 (diff | hist) Team:Washington/Notebook/protein gel
- 05:10, 15 October 2009 (diff | hist) Team:Washington/Notebook/NheI PstI
- 05:10, 15 October 2009 (diff | hist) Team:Washington/Notebook/colony PCR
- 05:10, 15 October 2009 (diff | hist) Team:Washington/Notebook/gene synthesis
- 05:09, 15 October 2009 (diff | hist) Team:Washington/Notebook/2mL purification
- 05:08, 15 October 2009 (diff | hist) Team:Washington/Notebook/Ni-column
- 05:08, 15 October 2009 (diff | hist) Team:Washington/Notebook/50mL purification
- 05:07, 15 October 2009 (diff | hist) Team:Washington/Notebook/Flow Cytometry
- 05:07, 15 October 2009 (diff | hist) Team:Washington/Notebook/Microscope
- 05:07, 15 October 2009 (diff | hist) Team:Washington/Notebook/protein gel
- 05:06, 15 October 2009 (diff | hist) Team:Washington/Notebook/NheI
- 04:59, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:52, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:17, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:17, 15 October 2009 (diff | hist) N Team:Washington/Notebook/NheI (New page: ==== Gene Assembly using the NheI site ==== #Start with first 23 coding nucleotides of gene, eg. 5'-atgcgtaaaggagaagaacttt...-3' #Replace the atg start codon with the XbaI site: 5'-TCTAGA-...)
- 04:15, 15 October 2009 (diff | hist) N Team:Washington/Notebook/NheI PstI (New page: ==== Gene Assembly using the NheI and PstI sites ==== #Start with first 23 coding nucleotides of gene, eg. 5'-atgcgtaaaggagaagaacttt...-3' #Replace the atg start codon with the XbaI site: ...)
- 04:14, 15 October 2009 (diff | hist) N Team:Washington/Notebook/colony PCR (New page: ==== Colony PCR ==== #Prepare one sterile 0.6mL tube with the following reaction mixture for each colony you intend to pick. ##5uL Qiagen Master Mix ##1uL 40uM VF2 ##1uL 40uM VR #Prepare o...)
- 04:13, 15 October 2009 (diff | hist) N Team:Washington/Notebook/gene synthesis (New page: ==== Gene Synthesis ==== #Generate Oligo's ##Go to: http://helixweb.nih.gov/dnaworks/ ##Set parameters ###Enter you job title and email ###Choose E.Coli Class II for codon frequency ###Se...)
- 04:10, 15 October 2009 (diff | hist) N Team:Washington/Notebook/2mL purification (New page: ==== Supernatant Protein Purification, 2mL ==== #Inoculate 50mL culture of TB with ~750uL overnight culture #Grow a 37c until OD600: 0.4 #Inoculate cells with IPTG so that the final concen...)
- 04:08, 15 October 2009 (diff | hist) N Team:Washington/Notebook/Ni-column (New page: ====Ni-column Set up==== #Transfer 2mL NiNTA beads (Quigen) to column #wash column with 10mL dH2O #Equillibrate column with 10mL running buffer (usually PBS) #To reuse column ##Wash with ...)
- 04:07, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:07, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:06, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 04:06, 15 October 2009 (diff | hist) N Team:Washington/Notebook/50mL purification (New page: ==== Supernatant Protein Purification, 50mL ==== #Inoculate 50mL culture of TB with ~750uL overnight culture #Grow a 37c until OD600: 0.4 #Inoculate cells with IPTG so that the final conce...)
- 04:03, 15 October 2009 (diff | hist) N Team:Washington/Notebook/Flow Cytometry (New page: ==== Flow Cytometry ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, p...)
- 04:00, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:57, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:55, 15 October 2009 (diff | hist) N Team:Washington/Notebook/Microscope (New page: ==== Microscope ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, place...)
- 03:54, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:53, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:52, 15 October 2009 (diff | hist) N Team:Washington/Notebook/protein gel (New page: ==== Protein Gel ==== # Set up overnights of parts 48-51 # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have all grown up uniformly start boilin...)
- 03:50, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:30, 15 October 2009 (diff | hist) Team:Washington/Notebook (→Gene Assemble using the NheI and PstI sites)
- 03:30, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 03:08, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 02:37, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 02:09, 15 October 2009 (diff | hist) Team:Washington/Notebook
- 20:40, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 19:44, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 17:55, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 17:41, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 17:41, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 16:45, 13 October 2009 (diff | hist) Team:Washington/Notebook
- 16:26, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:24, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:24, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:23, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:20, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:20, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:12, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:08, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:05, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:05, 13 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:08, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:07, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:06, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 02:05, 12 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:54, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:54, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:49, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:35, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:34, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:32, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:31, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:31, 8 October 2009 (diff | hist) Team:Washington/Team (→Stupendous Students)
- 04:30, 8 October 2009 (diff | hist) N File:Josef.jpg (top)
- 04:23, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 04:19, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:48, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:33, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:31, 8 October 2009 (diff | hist) File:Target structure.png (uploaded a new version of "Image:Target structure.png") (top)
- 03:27, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:27, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:25, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:24, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:23, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:20, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 03:15, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 01:35, 8 October 2009 (diff | hist) Team:Washington/Accomplishments
- 22:35, 7 October 2009 (diff | hist) Team:Washington/Accomplishments
- 19:37, 7 October 2009 (diff | hist) File:Main graphic.png (uploaded a new version of "Image:Main graphic.png")
- 17:11, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:10, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:04, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:03, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:00, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 17:00, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:59, 6 October 2009 (diff | hist) Team:Washington/Accomplishments
- 16:57, 6 October 2009 (diff | hist) Team:Washington
- 21:49, 4 October 2009 (diff | hist) Team:Washington/Accomplishments
- 21:48, 4 October 2009 (diff | hist) File:Display structure.png (uploaded a new version of "Image:Display structure.png") (top)
- 21:39, 4 October 2009 (diff | hist) File:Secretion structure.png (uploaded a new version of "Image:Secretion structure.png") (top)
- 21:31, 4 October 2009 (diff | hist) N File:Secretion structure.png
- 21:30, 4 October 2009 (diff | hist) N File:Display structure.png
- 21:30, 4 October 2009 (diff | hist) N File:Target structure.png
- 18:30, 4 October 2009 (diff | hist) Team:Washington
- 18:29, 4 October 2009 (diff | hist) Team:Washington
- 18:23, 4 October 2009 (diff | hist) Team:Washington
- 18:17, 4 October 2009 (diff | hist) Team:Washington
- 18:15, 4 October 2009 (diff | hist) Team:Washington
- 18:11, 4 October 2009 (diff | hist) N File:Main graphic.png (Graphic for main slide)