User contributions
From 2009.igem.org
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- 01:05, 21 October 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols/Digestion reaction (→Digestion Reaction)
- 01:05, 21 October 2009 (diff | hist) N Team:UNICAMP-Brazil/Protocols/Digestion reaction (New page: {{:Team:UNICAMP-Brazil/inc_topo}} Back to Protocols ==Digestion Reaction== 1ug DNA 1U enzyme 1 1U enzyme 2 0,2uL BSA 2uL Buffer 10X H2O up to 20uL {...)
- 01:03, 21 October 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols
- 23:56, 20 October 2009 (diff | hist) Talk:Jamboree/Social Event (→Program) (top)
- 14:22, 20 October 2009 (diff | hist) Talk:Jamboree/Social Event (→Program)
- 14:22, 20 October 2009 (diff | hist) Talk:Jamboree/Social Event (→Program)
- 11:17, 19 October 2009 (diff | hist) Talk:Jamboree/Social Event (→Program)
- 11:16, 19 October 2009 (diff | hist) Talk:Jamboree/Social Event (→Program)
- 20:24, 17 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/October 3 (→New biobricks - New strategy (pGEM))
- 20:20, 17 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 20:20, 17 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 16:52, 15 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/October 2 (→New biobricks - New strategy)
- 16:23, 13 October 2009 (diff | hist) Jamboree/Social Event (→The Members of the Committee)
- 15:29, 8 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/October 7 (→New biobricks - New strategy)
- 15:18, 8 October 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/October 8 (New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====New biobricks - New strategy ==== ''Raíssa'' {{:Team:UNICAMP-Brazil/...)
- 15:17, 8 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/October 7 (→New biobricks - New strategy)
- 15:07, 8 October 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/October 7 (New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard'''== ====New biobricks - New strategy ==== - We performed PCR of 7 colonies of each c...)
- 14:57, 8 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 14:56, 8 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 14:55, 8 October 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/October 6 (New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====New biobricks - New strategy==== - We inoculated Jen1orf-pGEM and Jen1promo...)
- 22:51, 1 October 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 22:50, 1 October 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/September 30 (New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====Screening - new biobricks==== - This morning we found some ''E. coli'' colo...)
- 19:51, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 29 (→New biobricks)
- 19:51, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols/Electroporation (top)
- 19:45, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 19:41, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 29 (→New biobricks)
- 19:40, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 29
- 19:40, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 29
- 19:36, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 19:36, 29 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Protocols/T4 DNA Ligase (New page: {{:Team:UNICAMP-Brazil/inc_topo}} Back to Protocols ==T4 DNA Ligase== - 5X Ligase Reaction Buffer - 4 μl - Insert: Vector Molar Ratio - 3:1 - Vect...) (top)
- 19:33, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 19:30, 29 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Protocols/CIAP Dephosphorylation (New page: {{:Team:UNICAMP-Brazil/inc_topo}} Back to Protocols ==CIAP Dephosphorylation== '''Protocol for Dephosphorylation of 5'-ends of DNA:''' 1. Dilute suffi...) (top)
- 19:24, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 19:22, 29 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Protocols/SAP Dephosphorylation (New page: {{:Team:UNICAMP-Brazil/inc_topo}} Back to Protocols ==SAP Dephosphorylation== '''Protocol for Dephosphorylation of 5'-ends of DNA:''' 1. Resuspend 1 p...) (top)
- 19:17, 29 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 19:14, 29 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/September 29 (New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} ==''' YeastGuard '''== ====Dephosphorylation - CIAP test==== - Few colonies were found this morning =( The test wa...)
- 14:54, 27 September 2009 (diff | hist) N File:DSC01169.JPG (top)
- 13:34, 20 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 18 (→Confirming the JEN1 orf, pJEN1, Lisozyme and pDLD biobricks)
- 13:33, 20 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 13 (→Lysozyme (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter))
- 13:32, 20 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 13:32, 20 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 13:31, 20 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 13:29, 20 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/September 13 (→Lysin (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter))
- 13:28, 20 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/September 18 (New page: ==''' YeastGuard '''== ====Confirming the JEN1 orf, pJEN1, Lisozyme and pDLD biobricks==== * We decided to make a bigger screening of our colonies to find that ones containing the biobri...)
- 12:26, 9 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 29 (→Lysozyme)
- 12:26, 9 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 29 (→Material)
- 12:24, 9 September 2009 (diff | hist) File:Chicken run 4.jpg (uploaded a new version of "Image:Chicken run 4.jpg") (top)
- 12:23, 9 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 29 (→Lysozyme)
- 12:22, 9 September 2009 (diff | hist) File:Chicken run 4.jpg (uploaded a new version of "Image:Chicken run 4.jpg")
- 12:18, 9 September 2009 (diff | hist) N File:Chicken run 4.jpg
- 18:36, 6 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 18:35, 6 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 18:34, 6 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 18:32, 6 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols/RNA isolation from 'Gallus gallus' spleen, blood and oviduct
- 18:31, 6 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Protocols/RNA isolation from 'Gallus gallus' spleen, blood and oviduct (New page: Isolation of RNA using Trizol Reagent (Invitrogen) 1.Homogenization a. Tissues Homogenize tissue samples in 1 ml of TRIzol® Reagent per 50-100 mg of tissue using a glass-Teflon® or p...)
- 18:30, 6 September 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 17:27, 5 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/September 5 (New page: == YeastGuard ==)
- 17:27, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 17:26, 5 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/August 27 (New page: == Unicamp-Brazil Team's meeting! == - Today we got together, talked about what we've donne and what we have to do in the next 50 days!! Lot of work!! {{:Team:UNICAMP-Brazil/inc_rodap...)
- 17:19, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 17:18, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Lab Notebooks)
- 17:16, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 21
- 17:15, 5 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/August 21 (New page: - Today we perfomed mini-prep from the RBS transformed ''E. coli''. The fragment syze was correct,we decided to digest it without purifying and it worked. - Afetr the RBS plasmid diges...)
- 17:09, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 17 (→YeastGuard: Trying to purify the RBS digested fragment)
- 17:08, 5 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/August 17 (New page: == YeastGuard: Trying to purify the RBS digested fragment == - The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band ...)
- 16:55, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 15 (→YeastGuard: RBS and CFP plasmid digestion)
- 16:54, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 15 (→YeastGuard: RBS and CFP plasmid's digestion)
- 16:53, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 15 (→YeastGuard: recovering BBa_J63002)
- 16:53, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 15
- 16:52, 5 September 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 15
- 16:49, 5 September 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/August 15 (New page: - Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP re...)
- 17:23, 15 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels (→PureLink™ Quick Gel Extraction Kit - Invitrogen)
- 17:22, 15 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels
- 17:20, 15 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels
- 17:19, 15 August 2009 (diff | hist) N Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels (New page: PureLink™ Quick Gel Extraction Kit - Invitrogen 1. Equilibrate a water bath or heat block to 50°C. 2. Excise the area of the gel containing your desired DNA fragment and minimize the a...)
- 17:12, 15 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 22:04, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks (→Checklist)
- 20:09, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→YeastGuard)
- 20:08, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→Coliguard - Genomic DNA Extraction for Hly Operon Manipulation)
- 19:43, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 8 (→YeastGuard - Mini-prep)
- 19:41, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 8 (→YeastGuard - Mini-prep)
- 18:53, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 18:51, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 18:50, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→Genomic DNA Extraction for Hly Operon Manipulation)
- 18:50, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→Genomic DNA Extraction for Hly Operon Manipulation)
- 18:49, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 18:47, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Protocols (→Protocols)
- 18:43, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 8 (→YeastGuard - Mini-prep)
- 18:43, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→Genomic DNA Extraction for Hly Operon Manipulation)
- 18:43, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→YeastGuard)
- 18:42, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 6 (→YeastGuard - Selection of Biobricks)
- 18:42, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 8 (→YeastGuard - Mini-prep)
- 18:26, 11 August 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/August 8 (New page: == YeastGuard - Mini-prep == - The protocol used is discribed at the section "Protocols" (nº 2). - The 6 over-night cultures had their plasmids extracted. - The agarose gel showed that...)
- 18:11, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 6 (→YeastGuard - Biobrick's selection)
- 18:09, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 6 (→YeastGuard - Biobrick selection)
- 18:09, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 6
- 18:05, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Notebooks/August 7 (→Genomic DNA Extraction for Hly Operon Manipulation)
- 17:53, 11 August 2009 (diff | hist) N Team:UNICAMP-Brazil/Notebooks/August 6 (Transformation)
- 12:29, 11 August 2009 (diff | hist) Team:UNICAMP-Brazil/Team (→Students)
- 23:31, 5 August 2009 (diff | hist) Team:UNICAMP-Brazil/Team (→Students)
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