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T4 DNA Ligase
- 5X Ligase Reaction Buffer - 4 μl
- Insert: Vector Molar Ratio - 3:1
- Vector Ends - 3-30 fmol
- Insert Ends - 9-90 fmol
- Total DNA - 0.01-0.1 μg
- T4 DNA Ligase - 0.1 unit
- Autoclaved distilled water to 20 uL
Temperature - 14°C
Time - 1 h
Protocol according to Invitrogen
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