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YeastGuard
Dephosphorylation - CIAP test
New biobricks
Raíssa and Taís
ColiGuard
Cre-Recombinase's and pSB1A3's purification
- Now that we obtained more Cre-Recombinase sample (see September 27th), we could proceed with Cre-Recombinase without ATG's biobrick construction.
- We ran an agarose gel with Cre-Recombinase's PCR product, as well as pSB1A3's miniprep sample. We then purified the target bands with Purelink's Quick Gel Extrection Kit, according to the manufacturer's protocol (Protocol 7).
Víctor
CeiB: Colony PCR
Apparently, this time we got transformed cells. We performed colony PCR with VF and VR primers in order to comprove the transformation. The CeaB transformation was wrong, because it didn’t show the right size band. We tried one more time the transformation. Otherwise, the CeiB transformation showed a possibly correct band size (~400). To confirm it, tomorrow we are going to make miniprep and PCR again.
Luige
Miniprep Results
Marcelo
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