Team:Aberdeen Scotland/WetLab

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==Wet Lab Procedures==
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=Wetlab: An Overview=
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Below is a list of some of the processes we went through in working on our project;
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[[Image:Wiring Diagram 3aberdeen20094.JPG|center|700px]]
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==Introduction==
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[[Cloning]]
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In order to work most efficiently we decided to split the wet lab work into 3 separate modules that can be constructed and tested individually, but are at the same time compatible to each other and can be transformed into a single cell upon completion.<br><br>
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[[Competency]]
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The first of these sub-projects is the AND gate of which Calum and Zuzana assumed charge of. In addition to this they also carried out the motility assays. <br><br>
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[[Digestion]]
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[[Image:AND gateAberdeen2009.JPG|center|600px]]
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[[Gel Preparation]]
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[[Mini Preps]]
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The second sub-project was the LacI-Latching mechanism as well as the Lysis function. This part of the project was mainly covered by Daniel and Risat.<br><br>
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[[Purification]]
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[[Image:LacI-latch+holinAberdeen2009.JPG|center|600px]]
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[[Transformation]]
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The third sub-project which was handled by Jennifer and Katriona included the Quorum Sensing in addition to a range of qualitative assays relating to the protein interaction outside of the cell.<br><br>
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[[Image:Quorum SensingAberdeen2009.JPG|center|400px]]
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<br><br>
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[[Image:Completed biobricks aberden 2009.jpg|center|700px]]
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<a href="https://2009.igem.org/Team:Aberdeen_Scotland/ethics"><img src="https://static.igem.org/mediawiki/2009/e/ed/Aberdeen_Left_arrow.png">&nbsp;&nbsp;Back to Ethics</a>
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<a href="https://2009.igem.org/Team:Aberdeen_Scotland/WetLab/andgate/overview">Continue to AND Gate Overview&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2009/4/4c/Aberdeen_Right_arrow.png"></a>
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Latest revision as of 09:18, 21 August 2009

University of Aberdeen iGEM 2009

Wetlab: An Overview

Wiring Diagram 3aberdeen20094.JPG

Introduction

In order to work most efficiently we decided to split the wet lab work into 3 separate modules that can be constructed and tested individually, but are at the same time compatible to each other and can be transformed into a single cell upon completion.

The first of these sub-projects is the AND gate of which Calum and Zuzana assumed charge of. In addition to this they also carried out the motility assays.

AND gateAberdeen2009.JPG


The second sub-project was the LacI-Latching mechanism as well as the Lysis function. This part of the project was mainly covered by Daniel and Risat.

LacI-latch+holinAberdeen2009.JPG


The third sub-project which was handled by Jennifer and Katriona included the Quorum Sensing in addition to a range of qualitative assays relating to the protein interaction outside of the cell.

Quorum SensingAberdeen2009.JPG



Completed biobricks aberden 2009.jpg


  Back to Ethics Continue to AND Gate Overview  

 

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