Team:BIOTEC Dresden/Methods SilverNano

From 2009.igem.org

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=== Silver Nano Particles - Methods ===
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=== Nucleation of silver nanoparticles on AG4 - Methods ===
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A ---- ml aliquot of the silver binding peptide ( sequence -----) was obtained from.... Two different experiments were setup using the following protocols.  
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There is an abundance of protocols for the formation of silver nanoparticles. After many attempts we decided to adapt method [1] employing sodium ascorbate as chemical reductant ti aid the formation process. the Pipeting scheme for the setup is shown below.
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*120 ul peptide (AG4)(8ug/ul)
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Protocol 1:
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*14 ul NaAsc. at 2mM
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A protocol that was initially used to identify metal binding peptides by was used to nucleate and grow silver nanoparticles[1].
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*120 ul peptide (8ug/ul)
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*14 ul HEPES buffer at pH 7.5
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*1.4 ul AgNO3 at 20mM
*1.4 ul AgNO3 at 20mM
*4.6 ul H2O
*4.6 ul H2O
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The solutions were mixed and incubated at room temperature for 24-48 hrs.
 
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Protocol 2:
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In the negative control AG4 was replaced by water. The absorption of the sample and the negative control were measured after 21 hours.
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Finally, the particles were analyzed by scanning electron microscopy.
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A method designed by Fromm and Wennemers [2] was used to form silver nanoparticles by using a chemical reducing agent, sodium ascorbate (NaAsc.)
 
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*120 ul peptide (8ug/ul)
 
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*14 ul NaAsc. at 2mM
 
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*1.4 ul AgNO3 at 20mM
 
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*4.6 ul H2O
 
== ==
== ==
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[1] R.Naik et al., Adv. Funct. Mater., 14, 25-30, 2004 <br>
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[1] K.Belser, K.Fromm, H.Wennemers et al., ''Angew. Chem. Int. Ed.'', 48, 3661-3664, 2009
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[2] K.Belser, K.Fromm, H.Wennemers et al., ''Angew. Chem. Int. Ed.'', 48, 3661-3664, 2009
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Latest revision as of 03:49, 22 October 2009

Nucleation of silver nanoparticles on AG4 - Methods

There is an abundance of protocols for the formation of silver nanoparticles. After many attempts we decided to adapt method [1] employing sodium ascorbate as chemical reductant ti aid the formation process. the Pipeting scheme for the setup is shown below.

  • 120 ul peptide (AG4)(8ug/ul)
  • 14 ul NaAsc. at 2mM
  • 1.4 ul AgNO3 at 20mM
  • 4.6 ul H2O

In the negative control AG4 was replaced by water. The absorption of the sample and the negative control were measured after 21 hours.

Finally, the particles were analyzed by scanning electron microscopy.


[1] K.Belser, K.Fromm, H.Wennemers et al., Angew. Chem. Int. Ed., 48, 3661-3664, 2009

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