Team:BIOTEC Dresden/Notebook2-3
From 2009.igem.org
(Difference between revisions)
(New page: {{:Team:BIOTEC_Dresden/NewTemplate}} {{:Team:BIOTEC_Dresden/NewTemplateEnd}}) |
|||
Line 1: | Line 1: | ||
{{:Team:BIOTEC_Dresden/NewTemplate}} | {{:Team:BIOTEC_Dresden/NewTemplate}} | ||
+ | '''14th September’09:''' | ||
+ | |||
+ | 1. Minipreps of GB05MN-Fosmid-BsdR pSC101-SpectR has been done and concentration is measured. | ||
+ | |||
+ | 2. A PCR for KanR2 was done using Kan-Fwd and Kan-Rev primers. | ||
+ | |||
+ | 3. Colonies are saved from both plates. | ||
+ | |||
+ | 4. A sequencing reaction has been set up for the positive clones of GB05 RhaFlpCmR with primerF and primerR primers using Taq polymerase. | ||
+ | |||
+ | 5. A enzyme digest for GB05MN FosBsdR-pSC101SpectR has been set. | ||
+ | |||
+ | '''17th September’09:''' | ||
+ | |||
+ | 1. A test digest run is performed with the minipreps from fosmids using HindIII enzyme. | ||
+ | |||
+ | '''18th September’09:''' | ||
+ | |||
+ | 1. Minipreps of pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR has been . | ||
+ | |||
{{:Team:BIOTEC_Dresden/NewTemplateEnd}} | {{:Team:BIOTEC_Dresden/NewTemplateEnd}} |
Latest revision as of 12:23, 21 October 2009
14th September’09:
1. Minipreps of GB05MN-Fosmid-BsdR pSC101-SpectR has been done and concentration is measured.
2. A PCR for KanR2 was done using Kan-Fwd and Kan-Rev primers.
3. Colonies are saved from both plates.
4. A sequencing reaction has been set up for the positive clones of GB05 RhaFlpCmR with primerF and primerR primers using Taq polymerase.
5. A enzyme digest for GB05MN FosBsdR-pSC101SpectR has been set.
17th September’09:
1. A test digest run is performed with the minipreps from fosmids using HindIII enzyme.
18th September’09:
1. Minipreps of pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR has been .