Team:BIOTEC Dresden/Notebook2-5

From 2009.igem.org

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'''28th September’09:'''
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1. A restriction digest of TT-FRT-GFP-BsdR has been done with PvuII.
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2. Colonies from pRhaFlp-CmR-kanR are sub-cultured in LB-Kan-Cm solution and incubated overnight.
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'''29th September’09:'''
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1. Cultures from pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR are inoculated and incubated for miniprep next day.
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2. The restriction digest of TT-FRT-GFP-BsdR is run on a gel and the insert is gel purified.
 +
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3. The restriction digest for Fosmid-SpecR is also run on a gel to check the quality of digest.
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'''30th September’09:'''
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 +
1. Minipreps of pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR have been done followed by a restriction digest with XhoI, HindIII, PvuII and BspEI respectively for concerned samples.
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2. The concentrations of the midiprep for pRhaFlp-CmR.KanR have been measured.
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{{:Team:BIOTEC_Dresden/NewTemplateEnd}}

Latest revision as of 12:30, 21 October 2009

28th September’09:

1. A restriction digest of TT-FRT-GFP-BsdR has been done with PvuII.

2. Colonies from pRhaFlp-CmR-kanR are sub-cultured in LB-Kan-Cm solution and incubated overnight.

29th September’09:

1. Cultures from pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR are inoculated and incubated for miniprep next day.

2. The restriction digest of TT-FRT-GFP-BsdR is run on a gel and the insert is gel purified.

3. The restriction digest for Fosmid-SpecR is also run on a gel to check the quality of digest.

30th September’09:

1. Minipreps of pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR have been done followed by a restriction digest with XhoI, HindIII, PvuII and BspEI respectively for concerned samples.

2. The concentrations of the midiprep for pRhaFlp-CmR.KanR have been measured.


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