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From 2009.igem.org

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Revision as of 18:09, 20 October 2009

iGEM 2009 Meeting April 22, 2009 9:40PM: Meeting begins in the SciLi Basement 9:43: iGEM account, Google groups account, Mathworks; email Neil for google group invitation! 9:45: Minoo and Eli are attending the Webinar 9:46: Michael gives presentation on Bacterial Pixel GFP, BFP, RGP components within a bacteria Wavelength promoters need to be cloned 10:00 Flora’s project: ability to detect cancer cells, then induce apoptosis Detecting target – binding site on breast cancer cells – using nanoparticles to identify Once cells are identified, induce apoptosis by bringing inducer molecule Flora will meet with them on Friday; report back; “Click Chemistry” 10:12: Indu & Minoo: prevention of allergies creating bi-functional protein – idenitify histamine & induce liver uptake Common allergies - too much histamine Tics secrete 3 binding histamine protein – have been cloned EV131 in mice – binds to histamine with high affinity Look into mechanism to fuse these 2 proteins 10:25: Will – unlike every other team on the planet let’s use RNA synthetic biology RNA strand with UTR and translated region Toxin trigger conformational change of RNA Make RNA that binds to RNA strand, blocking ribosome binding Another part of RNA has spot that binds to one molecule, allowing RNA strand to fall off Advantage: faster mechanism of gene regulation “Ribo-regulators” 10:38: Ahmad – specialized Safe Cell where dangerous cells will die if they escape Cell can only produce ATP on plate Uncouplers stop ATP synthesis Inhibitor of uncoupler – liver x receptor alpha in media – cell dies 10:48: Ashley – eat as much as you want! Leptin creates satiety signals, but obese individuals have large adipose, and large leptin; however, leptin receptors are not receptive Use leptin signal to break down fats directly Establish threshold leptin level 10:59: Stephanie – meningitis – bacteria has gene called lactate permease that when absent in mutant, mutant incapable of taking up lactose Uses lactose instead of glucose as primary energy source Bind something to lactose to prevent uptake Needs adhesins Build machine that recognizes bacteria then have gene expressed where lactose binding lactin => lactate broken down Proposed targeting this towards lactose intolerance 11:08: Eli – sphingosine and sphingosine 1-phosphate (signaling lipids) Spingosine is pro-apoptosis, anti-mitotic Spingosine 1-P is anti-apoptosis, mitogenic (overexpressed in cancer cells) Create switch that would induce cells to grow and die via ribo-regulator or regulation of transcription 11:16: Need to pick favorites 11:22: Everyone agrees on Bactopixel, Cancer-detection, and Histamine Possibility to implicate RNA regulators into each project Glue idea (ask Katrina) 11:27: Next meeting 9:30 to 11:30 Same Place TEAMS Steph & Will – ribo-regulators Will – Glue Minoo & Indu & Ashley – histamine Bactopixel – Michael & Eli Cancer – Flora & Ahmad

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