Team:Cambridge/Notebook/Week10

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Week 10

Monday

Wet Work

Threshold Devices

Confirmed successful transfer of 40 (pBad followed by GFP) into the low copy plasmid pSB3K3. Transformed 40 in pSB3K3 into arabinose strain.

Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.

Transformation of TOP10 with activator constructs 50, 51, 52 and the part B0015 in preparation for ligation to pigment parts.

Tuesday

Dry Work

Wet Work

Transformants from yesterday put into overnight culture.

Wednesday

Thursday

Friday

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