Team:Cambridge/Notebook/Week5

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Week 5

Monday

Dry Work

Violacein Synthesis

Used GeneDesigner from DNA2.0 to plan what we wanted synthesised, taking into account the different genes in the operon. This would be synthesised with codon optimisation for E. coli. All forbidden restriction sites have been removed (EcoRI, XbaI, SpeI, PstI, NotI)

New vio plan.PNG