Team:Cambridge/Project/Melanin
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'''Primer design''': | '''Primer design''': | ||
- | Primers were designed to isolate the melanin with the prefix and suffix | + | Primers were designed to isolate the melanin with the prefix and suffix. We are currently looking at adding a ribosome binding site to the the primers in order to produce a complete biobrick. |
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+ | '''PCR reactions''' | ||
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+ | Practical details to follow as soon as primers arrive. | ||
== Test colour changes by X == | == Test colour changes by X == |
Revision as of 10:11, 29 July 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Melanin Pigment
Introduction
Melanin is
Previous Work
The plasmid we used was from Duncan's stocks. We isolated it using miniprep to make stocks of our own before transforming it into a biobrick.
Action plan of our team
Our action plan is as follows:
- 1. Test for melanin production
- 2. Isolate MelA gene in biobrick form
- 3. Integrate Mel biobrick into system (e.g amplification of logic gate system)
Testing Activity of Melanin
Grew the plasmid in TOP10 E. coli
Pigment was clearly produced.
Constructing MelA biobrick
Primer design:
Primers were designed to isolate the melanin with the prefix and suffix. We are currently looking at adding a ribosome binding site to the the primers in order to produce a complete biobrick.
PCR reactions
Practical details to follow as soon as primers arrive.