Team:Cambridge/Project/Melanin

From 2009.igem.org

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Melanin is  
Melanin is  
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== Research by previous iGem teams ==
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== Previous Work ==
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Previous biobricks:
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The plasmid we used was from Duncan's stocks. We isolated it using miniprep to make stocks of our own before transforming it into a biobrick.
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! Registry Code
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! Sequence Description
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! Notes
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== Action plan of our team ==
== Action plan of our team ==
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Our action plan is as follows:
Our action plan is as follows:
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:1.  
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:1. Test for melanin production
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:2. Isolate MelA gene in biobrick form
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:3. Integrate Mel biobrick into system (e.g amplification of logic gate system)
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== Test activity of X ==
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== Testing Activity of Melanin ==
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'''Mapping of Biobrick X''':
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Grew the plasmid in TOP10 E. coli
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(image)
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[[Image:SDC10545.JPG]]
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== Construct composite Biobrick X ==
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Pigment was clearly produced.
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'''Plan mapping of X''':
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== Constructing MelA biobrick ==
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(image)
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'''Primer design''':
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Primers were designed to isolate the melanin with the prefix and suffix (
== Test colour changes by X ==
== Test colour changes by X ==

Revision as of 09:58, 29 July 2009


Melanin Pigment

Introduction

Melanin is

Previous Work

The plasmid we used was from Duncan's stocks. We isolated it using miniprep to make stocks of our own before transforming it into a biobrick.

Action plan of our team

Our action plan is as follows:

1. Test for melanin production
2. Isolate MelA gene in biobrick form
3. Integrate Mel biobrick into system (e.g amplification of logic gate system)

Testing Activity of Melanin

Grew the plasmid in TOP10 E. coli SDC10545.JPG

Pigment was clearly produced.

Constructing MelA biobrick

Primer design:

Primers were designed to isolate the melanin with the prefix and suffix (

Test colour changes by X

Characterisation of Biobricks and colour output

Test compatibility with other biobricks

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