Team:Cambridge/Project/Violacein

From 2009.igem.org

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(Violacein Pigment)
(Introduction)
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[[Image:Violacein pigment production.jpg]]
[[Image:Violacein pigment production.jpg]]
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As module 5 is Aqua, this gives us two different colour controlled by the four genes allowing the construction of logic gates.
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From P.R. August, T.H. Grossman, C. Minor, M.P. Draper, I.A. MacNeil, J.M. Pemberton, K.M. Call, d. Holt, and M. S. Osbourne, Sequence Analysis and Functional Characterization of the Violacein Biosynthetic Pathway from ''Chromobacterium violaceum'', J. Mol. Microbiol. Biotechnol. (2000) 2(4): 513-519. [http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf]
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Further, as module 5 is Aqua, controlling VioA, VioB, VioC, and VioD allows us to generate two different pigments, aqua and violet.
== Research by previous iGem teams ==
== Research by previous iGem teams ==

Revision as of 13:29, 4 August 2009


Violacein Pigment

Introduction

The Violacein pigment is produced from L-tryptophan via a pathway involving four enzymes, vioA-D. This scheme is shown below:

Violacein pigment production.jpg

From P.R. August, T.H. Grossman, C. Minor, M.P. Draper, I.A. MacNeil, J.M. Pemberton, K.M. Call, d. Holt, and M. S. Osbourne, Sequence Analysis and Functional Characterization of the Violacein Biosynthetic Pathway from Chromobacterium violaceum, J. Mol. Microbiol. Biotechnol. (2000) 2(4): 513-519. [http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf]

Further, as module 5 is Aqua, controlling VioA, VioB, VioC, and VioD allows us to generate two different pigments, aqua and violet.

Research by previous iGem teams

Previous biobricks:

Registry Code Team Sequence Description Notes

Action plan of our team

Our action plan is as follows:

1.

Test activity of X

Mapping of Biobrick X: (image)

Construct composite Biobrick X

Plan mapping of X:







(image)

Test colour changes by X

Characterisation of Biobricks and colour output

Test compatibility with other biobricks