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Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

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Violacein Pigment

Introduction

The Violacein pigment is produced from L-tryptophan via a pathway involving four enzymes, VioA-D. This scheme is shown below:

From P.R. August, T.H. Grossman, C. Minor, M.P. Draper, I.A. MacNeil, J.M. Pemberton, K.M. Call, d. Holt, and M. S. Osbourne, Sequence Analysis and Functional Characterization of the Violacein Biosynthetic Pathway from Chromobacterium violaceum, J. Mol. Microbiol. Biotechnol. (2000) 2(4): 513-519. [http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf]

Further, as module 5 is Aqua, controlling VioA, VioB, VioC, and VioD allows us to generate two different pigments, aqua and violet.

Our VioA-D genes are from Chromobacterium voilaceum.

Previous Work

Duncan Rowe provided us with the VioA-D operon on the pPSX-Vio+ plasmid, a very low copy number plasmid.

Action plan of our team

Our action plan is as follows:

1.

Test activity of X

Mapping of Biobrick X: (image)

Construct composite Biobrick X

Plan mapping of X:

(image)

Test colour changes by X

Characterisation of Biobricks and colour output

Test compatibility with other biobricks