Team:Cambridge/Research

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Research

Amplification

Response of the pBAD promoter to arabinose concentration

[http://www.ncbi.nlm.nih.gov/pubmed/7608087 Tight regulation, modulation and and high level expression by vectors containing the arabinose pBAD promoter, Guzman et al. 1995]

[http://www3.interscience.wiley.com/journal/121400517/abstract?CRETRY=1&SRETRY=0 A high throughput approach to promoter study using Green Fluorescent, Pigment Rao et al. 2008]

AraC changes conformation in the presence of arabinose to permit transcription. It is always bound in the region around pBAD:

[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCY-41S4XRM-G&_user=1495569&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=b367a1ca739f73db8c72c07a3ec217f0 Regulation of the L-Arabinose Operon in Escherichia Coli, Schleif 2000]

Pigments

Violacein

Most recent paper: Information about new colours and VioE: http://www3.interscience.wiley.com/cgi-bin/fulltext/112732008/PDFSTART

Older paper with first characterisation of the vio operon: http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf

The original paper, where they first isolated the operon: http://www.springerlink.com/content/q012rv3061829185/fulltext.pdf

Pseudomonas

Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO: [http://jb.asm.org/cgi/content/full/183/21/6454]


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