Keep track with what we did so far

(12.07.09)

This fourth week of wetlab we have done the following things

• Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part BBa_B0015). We created our first biobrick.

(12.07.09)

This first week of wetlab we have done the following things

• Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
• Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
• Ordered and received the primers needed for the PCR of LovTAP
• Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
• Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
• Fused the two BioBricks "LacI" and "RBS"
• Digested the LovTAP PCR products and RBS part