Keep track with what we did so far

(15.08.09)

Protocol of ligation was refined. !!! Inducible LOVTAP and Read out biobrick were created.!!! The system is completed. They need to be sequenced, characterized and submitted to the registery.

(08.08.09)

Problems in ligations.

(01.08.09)

This fourth week of wetlab we have done the following things

• Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part BBa_B0015). We created our first biobrick.
• The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
• Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.

(24.07.09)

This third week of wetlab we have done the following things

• Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
• Modeling part: Preliminar simulation was launched over the weekend.

(17.07.09)

This second week of wetlab we have done the following things

• Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
• Modeling part: Files required to launch simulations were created and analyzed.

(12.07.09)

This first week of wetlab we have done the following things

• Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
• Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
• Ordered and received the primers needed for the PCR of LovTAP
• Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
• Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
• Fused the two BioBricks "LacI" and "RBS"
• Digested the LovTAP PCR products and RBS part