Keep track with what we did so far

(01.08.09)

This fourth week of wetlab we have done the following things

• Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part BBa_B0015). We created our first biobrick.
• The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert

(12.07.09)

This first week of wetlab we have done the following things

• Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
• Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
• Ordered and received the primers needed for the PCR of LovTAP
• Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
• Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
• Fused the two BioBricks "LacI" and "RBS"
• Digested the LovTAP PCR products and RBS part