Team:EPF-Lausanne/Notebook

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(Notebook)
(Notebook)
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One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
LOVTAP is in a plasmid called pCal-n (see picture below):
LOVTAP is in a plasmid called pCal-n (see picture below):
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align="center"[[Image: pCAL-n.jpg|500px|thumb|left|alt text]]
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align="center"
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[[Image: pCAL-n.jpg|500px|thumb|left|alt text]]

Revision as of 15:42, 6 July 2009

Contents

Notebook

06.07.09

Wet lab:

LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight. One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. LOVTAP is in a plasmid called pCal-n (see picture below): align="center"

alt text


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