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Team:EPF-Lausanne/Notebook
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LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight. | LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight. | ||
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. | One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. | ||
| - | LOVTAP is in a plasmid called pCal-n (see picture below) | + | LOVTAP is in a plasmid called pCal-n (see picture below) |
{| | {| | ||
| - | |align="right | + | |align="right"| |
[[Image: pCAL-n.jpg|500px|thumb|left|alt text]] | [[Image: pCAL-n.jpg|500px|thumb|left|alt text]] | ||
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Revision as of 15:48, 6 July 2009
Contents |
Notebook
06.07.09
- Wet lab:
LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight. One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. LOVTAP is in a plasmid called pCal-n (see picture below)
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