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Team:EPF-Lausanne/Notebook
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=Notebook= | =Notebook= | ||
===06.07.09=== | ===06.07.09=== | ||
| - | + | ;Wet lab: | |
LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight. | LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight. | ||
| - | One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. | + | <br>One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. |
LOVTAP is in a plasmid called pCal-n (see picture below): | LOVTAP is in a plasmid called pCal-n (see picture below): | ||
[[Image: pCAL-n.jpg|500px|thumb|center|pCal-n plasmid]] | [[Image: pCAL-n.jpg|500px|thumb|center|pCal-n plasmid]] | ||
| - | + | ;Cloning strategy: | |
| + | Four plasmids were designed: | ||
| + | <br>one taking promoter T7 | ||
{| style="color:#1b2c8a;background-color:#0c9;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | {| style="color:#1b2c8a;background-color:#0c9;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
Revision as of 15:51, 6 July 2009
Contents |
Notebook
06.07.09
- Wet lab
LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight.
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
LOVTAP is in a plasmid called pCal-n (see picture below):
- Cloning strategy
Four plasmids were designed:
one taking promoter T7
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