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Team:EPF-Lausanne/Notebook
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| - | Four | + | Four primers were designed to amplify: |
| - | + | #Promoter T7, RBS, CBP and LOVTAP | |
| + | #RBS, CBP and LOVTAP | ||
| + | #CBP and LOVTAP | ||
| + | #LOVTAP | ||
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Revision as of 15:54, 6 July 2009
Contents |
Notebook
06.07.09
- Wet lab
LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight.
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
LOVTAP is in a plasmid called pCal-n (see picture below):
- Cloning strategy
Four primers were designed to amplify:
- Promoter T7, RBS, CBP and LOVTAP
- RBS, CBP and LOVTAP
- CBP and LOVTAP
- LOVTAP
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