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Team:EPF-Lausanne/Notebook
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<br>Some comments on the plasmid: | <br>Some comments on the plasmid: | ||
| - | - CBP is a small peptide with which we could purify LOVTAP protein | + | <br>-CBP is a small peptide with which we could purify LOVTAP protein |
| - | - | + | <br>-Thrombin target is a nucleotidic sequence that can be recognized by thrombin a peptidase. This peptidase will cut CBP once lOVTAP is purified |
;Cloning strategy: | ;Cloning strategy: | ||
| - | Four primers were designed to amplify: | + | Four forward primers were designed to amplify: |
#Promoter T7, RBS, CBP and LOVTAP | #Promoter T7, RBS, CBP and LOVTAP | ||
#RBS, CBP and LOVTAP | #RBS, CBP and LOVTAP | ||
Revision as of 16:02, 6 July 2009
Contents |
Notebook
06.07.09
- Wet lab
LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight.
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
LOVTAP is in a plasmid called pCal-n (see picture below):
Some comments on the plasmid:
-CBP is a small peptide with which we could purify LOVTAP protein
-Thrombin target is a nucleotidic sequence that can be recognized by thrombin a peptidase. This peptidase will cut CBP once lOVTAP is purified
- Cloning strategy
Four forward primers were designed to amplify:
- Promoter T7, RBS, CBP and LOVTAP
- RBS, CBP and LOVTAP
- CBP and LOVTAP
- LOVTAP
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