Notebook

06.07.09

Wet Lab

Cloning Strategy

People in the lab

Tu, Heidi, Rafael, Basile, Nath

07.07.09

Remark for the notebook

First, it's great you already started to use the wiki and customised the menu!
Then I think we should add the name of the peoples who worked on each part of a process (or at least present the same day). It would allow easy team transitions.
For the wiki in general, as you did in this page, it is much better not to use html tags.
We will have a meeting for the modeling on tuesday, I will come to the lab before.

Wet Lab

We have to grow the 3 strains generously sent by Tom Beatty

The three strains are :

• R.Palustris CEA001 (wild type) ; should be grown on LB medium only
• R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
• E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)

The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)

pUC19 plasmid

We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.

Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.

Cloning Strategy

To design plasmids : software Vector NTI

People in the lab

Tu, Rafael, Nath, Heidi, Basile

08.07.09